Fig. 4
From: Microcavity-assisted cloning (MAC) of hard-to-clone HepG2 cell lines: cloning made easy
Proof-of-concept of the MAC method for CRISPR/Cas9 ko mutants. Western blot to detect GSTA1 in 19 clones obtained with MAC method. Out of 19 clones screened, 11 were GSTA1 knockouts confirming the ability of the method to isolate clonal cell populations. L = ladder (25 kDa)