Fig. 1

Results of different exercised fed-batch cultivations; the color code is: control run A , control run B , screening 1 , screening 2 . A: Applied feeding strategies. For the control runs an exponential feeding profile for 21 h was used, before a constant feed was applied. In screening 1 and 2 the effects of a different constant specific substrate uptake rate (q_s) were examined (exponential feeding). B: Trend of the optical density measured at 600 nm (OD600) for the tested fed-batch cultivations. C: Specific substrate uptake rates (q_s) normalized to the maximal substrate uptake rate of the cultivated strain. D: Biomass formation rate (r_x). E: Trend of the obtained protease concentration over time, normalized to the titer achieved at the harvesting time point of control run A. F: Specific product formation rates (q_p) normalized to the maximal q_p achieved in the control run A at the end of the fermentation